The Sperm Chromatin Structure Assay (SCSA®) and other sperm DNA fragmentation tests for evaluation of sperm nuclear DNA integrity as related to fertility
نویسندگان
چکیده
منابع مشابه
Environmental toxicants cause sperm DNA fragmentation as detected by the Sperm Chromatin Structure Assay (SCSA).
Studies over the past two decades have clearly shown that reproductive toxicants cause sperm DNA fragmentation. This DNA fragmentation can usually be detected prior to observing alterations of metaphase chromosomes in embryos. Thus, Sperm Chromatin Structure Assay (SCSA)-detected DNA damage is viewed as the molecular precursor to later gross chromosome damage observed under the light microscope...
متن کاملSperm nuclear DNA fragmentation and chromatin structure in one-day-old ejaculated sperm
OBJECTIVE To evaluate sperm nuclear DNA fragmentation and chromatin structure after 18 hours'incubation at room temperature. METHODS Twenty-eight male partners who participating IVF treatment were prospectively included in this study. Ejaculated sperm count and motility were assessed. The sperm was then immediately processed by the conventional swim-up method. After utilization of some of the...
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The Sperm Chromatin Structure Assay (SCSA®) is the pioneering sperm DNA fragmentation assay that precisely measures the percent of sperm in a semen sample that have Sperm DNA Damage (SDD), a negative factor for successful pregnancy. The SCSA® is a rapid, dual parameter, computer driven assay with diligent unbiased flow cytometer measurements on five to ten thousand sperm per sample providing ri...
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The traditional semen analysis is a cornerstone in diagnosis and treatment of male fertility (World Health Organisation (WHO), 1999, 2010). The sperm parameters concentration, motility and morphology are, however, claimed to be poor predictors of a male ́s fertility status, this in natural conception (Bonde et al., 1998; Guzick et al., 2001) as well as in assisted reproductive techniques (ART) (...
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Background: One of the main causes of male infertility is the negative effects of oxidative stress. Follicle-stimulating hormone (FSH) plays an essential role in spermatogenesis, as well as in the maintenance of sperm DNA integrity. This study aimed to determine whether the recombinant human follicle-stimulating hormone (rhFSH) treatment of sperm parameters could positively affect sperm DNA and...
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ژورنال
عنوان ژورنال: Animal Reproduction Science
سال: 2016
ISSN: 0378-4320
DOI: 10.1016/j.anireprosci.2016.01.017